{"title":"Antibody Panels and Kits","description":"","products":[{"product_id":"invitrogen™-elisa-tmb-stabilized-chromogen","title":"eBioscience™ Human\/Non-Human Primate Regulatory T Cell Staining Kit #1","description":"\u003ch3 id=\"qa_spec_label_includes_label\"\u003eIncludes\u003c\/h3\u003e\n\u003cp id=\"qa_spec_content_include_desc\"\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eFlow Cytometry Staining Buffer\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(cat. 00-4222): 200mL, store at 2-8°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eFixation\/Permeabilization Concentrate (4X)\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(cat. 00-5123): 30mL, store at 2-8°C. Avoid agitation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eFixation\/Permeabilization Diluent\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(cat. 00-5223): 100mL, store at 2-8°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003ePermeabilization Buffer (10X)\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(cat. 00-8333): 100mL, store at 2-8°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eNormal Rat Serum\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(cat. 24-5555): 100 μL, store at 2-8°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eAnti-Human CD4 FITC\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(clone OKT4, cat. 11-0048): 25 tests, store at 2-8°C. Light-sensitive material.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eAnti-Human CD25 APC\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(clone BC96, cat. 17-0259): 25 tests, store at 2-8°C. Light-sensitive material.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eAnti-Human Foxp3 PE\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e(clone PCH101, cat. 12-4776): 25 tests, store at 2-8°C. Light-sensitive material.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eRat IgG2a K Isotype Control PE\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(cat. 12-4321): 25 tests, store at 2-8°C. Light-sensitive material.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp id=\"qa_spec_content_teasertext\"\u003eAntibody Staining Kit\u003c\/p\u003e\n\u003cdiv class=\"qa_spec_content_productfeatures\"\u003eThis Human\/Non-human Primate Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human, rhesus, cynomolgus, or chimpanzee peripheral blood cells. This kit may also be used to identify regulatory T cells in baboons, using the CD25 and Foxp3 monoclonal antibodies, however, the monoclonal antibody for CD4 that is included in this kit does not crossreact with baboon.\u003cbr\u003e\u003cbr\u003eThe OKT4 monoclonal antibody reacts with human, rhesus, cynomolgus, and chimpanzee CD4, a 59 kDa glycoprotein found on the surface of the majority of thymocytes, a subset of mature T cells (T helper cells), and at lower levels on monocytes. The BC96 monoclonal antibody reacts with human, rhesus, cynomolgus, chimpanzee, and baboon CD25 (also known as interleukin-2 receptor alpha, IL-2R alpha), a 55 kDa surface protein expressed by early progenitors of T cells and B cells, by mature, activated T cells and B cells, and at constitutively high levels on regulatory T cells. The PCH101 monoclonal antibody reacts with the amino terminus of human, rhesus, cynomolgus, chimpanzee, and baboon Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead\/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.\u003c\/div\u003e","brand":" Assignment Center","offers":[{"title":"Default Title","offer_id":46643242762402,"sku":null,"price":722.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0708\/6525\/6610\/files\/primary-antibody-image.jpg-650.webp?v=1772747480"},{"product_id":"invitrogen™-elisa-tmb-stabilized-chromogen-1","title":"Invitrogen™ ELISA TMB Stabilized Chromogen","description":"\u003cp id=\"qa_spec_content_teasertext\"\u003eFor use with all sandwich ELISAs which utilize Horseradish Peroxidase (HRP) as detecting enzyme\u003c\/p\u003e\n\u003cdiv class=\"qa_spec_content_productfeatures\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFormulation: Liquid\u003c\/li\u003e\n\u003cli\u003eAvailable in two sizes\u003c\/li\u003e\n\u003cli\u003eDetection method: Colormetric\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eBuild Your Own Immunoassay, Cell Analysis, Immunoassays\u003c\/p\u003e\n\u003c\/div\u003e","brand":" Assignment Center","offers":[{"title":"Default Title","offer_id":46643253215394,"sku":null,"price":95.65,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0708\/6525\/6610\/files\/SB01_650x600.jpg-650_65d87c55-04c0-4e48-8ca5-0b6b9bfffa92.webp?v=1772747758"},{"product_id":"bd-compbead-anti-mouse-ig-κ-negative-control-particles-set","title":"BD CompBead Anti-Mouse Ig, κ\/Negative Control Particles Set","description":"\u003cp id=\"qa_spec_content_teasertext\"\u003eUsed to optimize fluorescence compensation settings for multicolor flow cytometric analyses. BD™ Anti-Mouse Ig, κ\/Negative Control Particles Set includes two sets of polystyrene microparticles to distinctly stain positive and negative populations, especially when using tandem dye conjugates.\u003c\/p\u003e\n\u003cdiv class=\"qa_spec_content_productfeatures\"\u003e\n\u003cp\u003eThe set provides two populations of microparticles:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBD™ CompBeads Anti-Mouse Ig, κ—Binds any mouse κ light chain-bearing immunoglobulin\u003c\/li\u003e\n\u003cli\u003eBD™ CompBeads Negative Control—Has no binding capacity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen mixed together with a fluorochrome-conjugated mouse antibody, the CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software.\u003c\/p\u003e\n\u003cp\u003eSince the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra).\u003c\/p\u003e\n\u003cp\u003eUse of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.\u003c\/p\u003e\n\u003c\/div\u003e","brand":" Assignment Center","offers":[{"title":"Default Title","offer_id":46643257114786,"sku":null,"price":295.5,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0708\/6525\/6610\/files\/F867063.jpg-650.webp?v=1772747958"},{"product_id":"invitrogen™-elisa-assay-buffer-5x","title":"Invitrogen™ ELISA Assay Buffer (5X)","description":"\u003cp id=\"qa_spec_content_teasertext\"\u003eFor use with antibody pairs (ELISA)\u003c\/p\u003e\n\u003cdiv class=\"qa_spec_content_productfeatures\"\u003e\n\u003cp\u003e\u003cb\u003eConcentrated 5X Assay Buffer for Antibody Pairs is intended to be used as:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBlocking reagent for ELISA plates\u003c\/li\u003e\n\u003cli\u003eDiluent for ELISA standards and samples\u003c\/li\u003e\n\u003cli\u003eDiluent for the detection antibody\u003c\/li\u003e\n\u003cli\u003eDiluent for the HRP conjugate\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eBuild Your Own Immunoassay, Cell Analysis, Immunoassays\u003c\/p\u003e\n\u003c\/div\u003e","brand":" Assignment Center","offers":[{"title":"Default Title","offer_id":46643259179170,"sku":null,"price":234.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0708\/6525\/6610\/files\/DS98200_650x600.jpg-650.webp?v=1772748073"},{"product_id":"bd-phosflow™-human-monocyte-nk-cell-activation-kit","title":"BD Phosflow™ Human Monocyte\/NK Cell Activation Kit","description":"\u003ch3 id=\"qa_spec_label_includes_label\"\u003eIncludes\u003c\/h3\u003e\n\u003cp id=\"qa_spec_content_include_desc\"\u003eAntibody cocktail, fluorochrome-labled antibodies to six key phosphoproteins, optimized protocols and buffers\u003c\/p\u003e\n\u003cp id=\"qa_spec_content_teasertext\"\u003eStudy monocyte and NK cell signaling events without prior isolation of cells from whole blood\u003c\/p\u003e\n\u003cdiv class=\"qa_spec_content_productfeatures\"\u003e\n\u003cp\u003eComplete system for the determination of the activation status of six different key phosphoproteins in B cells, monocytes, NK cells, and T cells using whole blood samples.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eEvaluate:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePhosphorylation status information on the intracellular MAPK and JAK\/STAT signaling pathway\u003c\/li\u003e\n\u003cli\u003eResponses transduced by treated human monocytes and NK cells\u003c\/li\u003e\n\u003cli\u003eSignaling events in T and B-cell populations\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cb\u003eBenefits:\u003c\/b\u003e\n\u003cul\u003e\n\u003cli\u003eLyophilized positive and negative control cells to increase confidence and standardization\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eFlow cytometry\u003c\/p\u003e\n\u003c\/div\u003e","brand":" Assignment Center","offers":[{"title":"Default Title","offer_id":46643262095522,"sku":null,"price":2080.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0708\/6525\/6610\/files\/F873768.jpg-650.webp?v=1772748179"}],"url":"https:\/\/assignment-center.myshopify.com\/collections\/antibody-panels-and-kits.oembed","provider":" Assignment Center","version":"1.0","type":"link"}